Isolation and Molecular Identification of Two Fungal Strains Capable of Degrading Hydrocarbon Contaminants on Saudi Arabian Environment

نویسندگان

  • Amr A. El Hanafy
  • Saleh A. Mohamed
  • Saleh Mohamed Saleh Al - Garni
  • S. M. Sabir
  • Osama A. H. Abu Zinadah
  • Mohamed Morsi Ahmed
چکیده

In the vicinity of red sea about 15 fungi species were isolated from oil contaminated sites. On the basis of aptitude to degrade the crude oil and DCPIP assay, two fungal isolates were selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2 and amplicon pyrosequencing studies of fungal diversity revealed that these strains belong to Penicillium and Aspergillus species. Two strains that proved to be the most efficient in degrading crude oil was Aspergillus niger (54%) and Penicillium commune (48%) Subsequent to two weeks of cultivation in BHS medium the degradation rate were recorded by using spectrophotometer and GC-MS. Hence, it is cleared that these fungal strains has capability of degradation and can be utilize for cleaning the Saudi Arabian environment. Keywords—Fungal strains, hydrocarbon contaminants, molecular identification, biodegradation, GC-MS.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and Identification of Phenanthrene-degrading Bacteria and Increasing the Biodegrading Ability by Synergistic Relationship

Background: Polycyclic aromatic hydrocarbons are a large group of oil contaminants with carcinogenic, mutagenic and teratogenic effects. The release of these compounds in soil destroys animals, plants and microbial diversity and has several negative impacts on physical properties of the soil including the destruction of soil aggregates reduction in pores, and increase in soil bulk density....

متن کامل

Enhancing Bioremediation of Saudi Desert Soils Polluted with Hydrocarbons

List of Abbreviations CIIAPTER ONE General Introduction Pollution of Saudi Arabian soil with crude oil Chemistry and biodegradability of petroleum hydrocarbon Environmental fate of petroleum Factors influencing petroleum hydrocarbon biodegradation Distribution of petroleum-utilizing microorganisms in the environment Biological degradation of petroleum hydrocarbon Bacterial degradation of oil Fu...

متن کامل

Isolation and Identification of Pyrene-degrading Bacteria from Soils around Landfills in Shiraz and Their Growth Kinetic Assay

Background & Objectives: Pyrene is a kind of carcinogen hydrocarbon in environment and one of the top 129 pollutants as ranked by the U.S.Environmental Pretection Agency (USEPA). Today's commodious method that is considered by many researchers is the use of microorganisms to degrade these compounds from the environment. The goal of this research is separation and identification of the indigenou...

متن کامل

Isolation and identification of crude oil degrading bacteria from mangrove forests of Khamir and Minab ports located at north of Persian Gulf

Mangrove forests are coastal ecosystems that found worldwide in tropical and subtropical zones. Oil spill causes damage to these marine ecosystems. The objective of this study is study the oil-degrading bacteria in some mangrove forests that located at Persian Gulf. In this study sediments and plant materials were collected from mangrove forests at Persian Gulf (Minab and Khamir ports). Oil-deg...

متن کامل

Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils

Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015